The development of fluorescence lifetime imaging system
نویسندگان
چکیده
منابع مشابه
Tomographic fluorescence lifetime imaging
Fluorescence lifetime imaging microscopy (FLIM) is a well-established technique (Bastiaens and Squire 1999; Berezovska et al. 2003; Selvin 2000; Vogel et al. 2006) that combines microscopic techniques with time-resolved detection to provide high-resolution lifetime images of thin tissue sections. This chapter concerns the in vivo time domain imaging of fluorescent contrast agents embedded in de...
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We report a fluorescence lifetime imaging technique that uses the time integrated response to a periodic optical excitation, eliminating the need for time resolution in detection. A Dirac pulse train of variable period is used to probe the frequency response of the total fluorescence per pulse leading to a frequency roll-off that is dependent on the relaxation rate of the fluorophores. The tech...
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We report the development and detailed calibration of a multiphoton fluorescence lifetime imaging system (FLIM) using a streak camera. The present system is versatile with high spatial (~0.2 μm) and temporal (~50 psec) resolution and allows rapid data acquisition and reliable and reproducible lifetime determinations. The system was calibrated with standard fluorescent dyes and the lifetime valu...
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There is an increasing interest in the application of fluorescence lifetime imaging (FLIM) for medical diagnosis. Central to the clinical translation of FLIM technology is the development of compact and high-speed clinically compatible systems. We present a handheld probe design consisting of a small maneuverable box fitted with a rigid endoscope, capable of continuous lifetime imaging at multi...
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ژورنال
عنوان ژورنال: Seibutsu Butsuri
سال: 2003
ISSN: 0582-4052,1347-4219
DOI: 10.2142/biophys.43.s17_4